PKMYT1 (phospho T495) polyclonal antibody
产品名称： PKMYT1 (phospho T495) polyclonal antibody
英文名称： PKMYT1 (phospho T495) polyclonal antibody
产品编号： PAB0542
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152
- 传真 : +886-2-8751-1186
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of PKMYT1.
- Immunogen:
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T495 of human PKMYT1.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Dot Blot (1:500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Keratinocyte G2/M growth arrest by 1,25-dihydroxyvitamin D3 is caused by Cdc2 phosphorylation through Wee1 and Myt1 regulation.
Dai X, Yamasaki K, Yang L, Sayama K, Shirakata Y, Tokumara S, Yahata Y, Tohyama M, Hashimoto K.J Invest Dermatol. 2004 Jun;122(6):1356-64.
- 2.
- Identification of a consensus motif for Plk (Polo-like kinase) phosphorylation reveals Myt1 as a Plk1 substrate.
Nakajima H, Toyoshima-Morimoto F, Taniguchi E, Nishida E.J Biol Chem. 2003 Jul 11;278(28):25277-80. Epub 2003 May 8.
- 3.
- The p53-inducible TSAP6 gene product regulates apoptosis and the cell cycle and interacts with Nix and the Myt1 kinase.
Passer BJ, Nancy-Portebois V, Amzallag N, Prieur S, Cans C, Roborel de Climens A, Fiucci G, Bouvard V, Tuynder M, Susini L, Morchoisne S, Crible V, Lespagnol A, Dausset J, Oren M, Amson R, Telerman A.Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2284-9. Epub 2003 Feb 26.
- Applications
- Western Blot (Cell lysate)
- The PKMYT1 (phospho T495) polyclonal antibody (Cat # PAB0542) is used in Western blot to detect Phospho-PKMYT1-T495 in HL-60 (left) and SK-BR-3 (right) cell lysates
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with PKMYT1 (phospho T495) polyclonal antibody (Cat # PAB0542) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Entrez GeneID:
- 9088
- Protein Accession#:
- NP_004194;Q99640
- Gene Name:
- PKMYT1
- Gene Alias:
- DKFZp547K1610,FLJ20093,MYT1
- Gene Description:
- protein kinase, membrane associated tyrosine/threonine 1
- Omim ID:
- 602474
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
- Other Designations:
- membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase,protein kinase Myt1
- Interactome
- Gene Pathway