Biotin-16-dCTP生物素标记物-生物分子-试剂-生物在线
武汉博欧特生物科技有限公司
Biotin-16-dCTP生物素标记物

Biotin-16-dCTP生物素标记物

商家询价

产品名称： Biotin-16-dCTP生物素标记物

英文名称： Biotin-16-dCTP

产品编号： orb64049

产品价格： 7150元

产品产地： UK

品牌商标： Biorbyt

更新时间： 2026-03-19T16:18:36

使用范围： null

规格 价格
5×200ul 7150.0
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Biotin-16-dCTP

SKU: orb64049
 

Description

Biotin-16-dCTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detec-tion of the Biotin moiety is ensured by a 16-atom linker attached to the C5 position of cytidine. Recommended Biotin-16-dCTP/dCTP ratio for PCR: 50% Biotin-16-dCTP/50% dCTP.
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Customer Validated Data

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The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this antibody works for your specific application or species, even if we haven't tested it ourselves.
Success by Application
 
 
 
 
 
 
 
Reactivity Distribution
 
 

Latest Experiments

5 Results
Dilution
Sample
Summary
Klenow fill-in reaction
Bacteria
33 μM
Linearized plasmid DNA (generated by digestion with BspQI restriction enzyme)
The primary application was to biotinylate...
 
sSTRIDE (sodium iodide-based transferase-independent DNA end-labeling)
Human
5 μM
Human cell lines (specifically hTERT RPE-1 cells) grown on coverslips
The primary application was in the...
 
Nascent Chromatin Capture (NCC) assay
Human
0.5 μM
Adherent human cell lines (specifically HLF cells, a hepatocellular carcinoma cell line)
The primary application was in the...
 
ARTR-seq (Assay of Reverse Transcription-based RBP binding site sequencing)
Human
0.05 mM
Fixed and permeabilized cells (HeLa, HepG2), as well as mouse embryo tissue sections
The primary application was in the...
 
Led-Seq (Lead Sequencing)
Bacteria
150 μM
Total RNA isolated from E. coli cells (treated with or without lead(II) acetate)
The primary application was in the...
 

Images & Validation

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Application Notes
Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase I; Primer Extension with Klenow exo-; 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) ; Reverse Transcription with MMLV Reverse Transcriptase. Spectroscopic Propertie: λmax 294 nm, ε 9.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5).
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Key Properties

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MW Theoretical MW: 944.78 g/mol (free acid); Detected MW: 944.23 g/mol (free acid)
Purity ≥ 95% (HPLC)
Formula C32H51N8O17P3S (free acid)

Storage & Handling

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Storage store at -20 °C
Expiration Date 12 months from date of receipt.
Disclaimer For research use only

Alternative Names

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Biotin-16-Propargylamino-dCTP, γ-[N-(Biotin-6-amino-hexanoyl-6-aminobutanoyl)]-5-(3-propargylamino)-2'-deoxycytidine-5'-triphosphate, Triethylammonium salt