CollagenaseⅡ  胶原酶Ⅱ来源于溶组织梭菌

一、产品基本信息：

货号：C8150

产品名称：胶原酶Ⅱ

英文名称： CollagenaseⅡ ；Clostridiopeptidase A 

别名：骨胶原酶；胶原蛋白酶；胶原蛋白水解酶

CAS号：9001-12-1 

级别：BR

外观：棕色或棕褐色结晶性粉末

来源：溶组织棱状芽胞杆菌

说明：胶原酶Ⅱ适用于肝脏，骨，甲状腺，心脏和唾液腺组织细胞的分离。

活力：≥125CDU/mg solid (CDU=collagen digestion units)

活力定义：One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at pH 7.5 at 25 °C in the presence of calcium ions。One neutral protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole tyrosine per 5 hr at pH 7.5 at 37℃。One clostripain unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25℃ in the presence of DTT

R： 36/37/38-42 S： 22-24-26-36/37

储存条件：-20℃

二、产品用途：

Most preparations contain collagenase, non-specifc protease, and clostripain activities. These enzymes act synergistically to degrade collagen and other intercellular material. Crude Collagenases are suitable for isolation of cells from many types of animal tissue. All are evaluated for their enzymatic activities. The optimal concentration for use and digestion time of tissue needs to be empirically determined. In addition, Sigma-Aldrich also offers collagenases which are tested for suitability for release of cells from specified tissues (release of rat pancreatic islets, rat epididymal adipocytes, and rat hepatocytes.

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