DNA试剂盒-细胞生物学检测-试剂-生物在线
DNA试剂盒

DNA试剂盒

商家询价

产品名称: DNA试剂盒

英文名称: Quick Blunting Kit

产品编号: E1201L

产品价格: 0

产品产地: 美国

品牌商标: NEB

更新时间: null

使用范围: null

北京百奥创新科技有限公司
  • 联系人 :
  • 地址 : 北京市海淀区创客小镇
  • 邮编 :
  • 所在区域 : 北京
  • 电话 : 133****0421
  • 传真 : 01057187533
  • 邮箱 : customer@bestopbio.com;3007606172@qq.com

DNA is blunted using T4 DNA Polymerase (NEB #M0203) which has both 3 → 5 exonuclease activity and 5 → 3 polymerase activity. T4 Polynucleotide Kinase (NEB #M0201) is included in the enzyme mix for phosphorylation of the 5 ends of blunt-ended DNA for subsequent ligation into a cloning vector. This kit is optimized for blunting up to 5 g of DNA in a single reaction. 
Blunt Enzyme Mix supplied in: 100 mM KCl, 10 mM Tris-HCl(pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 0.1% Triton X-100 and 50% Glycerol.